@article{4e108f8a7d0246d99290ee90a322422f,
title = "Understanding the Influence of Water-Soluble Compounds from Unpretreated Corn Stover Pellets on Enzymatic Hydrolysis of Cellulose",
abstract = "Aqueous extractives are minor and nonstructural compounds in biomass that can be extracted using solvents, while contributing significantly to lignocellulose characteristics. Yet their roles in enzyme-mediated processing of lignocellulosic biomass remain elusive. Here, we examine the composition and features of extractives derived from untreated pelleted corn stover as well as their effects on enzymatic hydrolysis. Unlike the observations described in previous reports, we find that water-extractable material improves the enzymatic hydrolysis of extractive-free stover by 67\% with a glucose yield increase from 12 to 20\% with 6 FPU cellulase per gram of glucan, whereas the enzyme activities are diminished when using microcrystalline cellulose (MCC) as a substrate. The different behavior of corn stover and MCC is likely attributed to the presence of lignin, which may interact with inhibitory compounds, such as phenolics, mitigating the detrimental impacts of soluble inhibitors, insoluble lignin, or both. These findings advance our fundamental understanding of the intrinsic behavior of extractives and help us to optimize the schemes for efficient and cost-competitive enzymatic conversion of lignocellulose.",
keywords = "enzyme activity, extractives, inhibition, lignin interaction, pelleted corn stover, phenolics",
author = "Xueli Chen and \{Freitas dos Santos\}, \{Antonio C.\} and \{Ramirez Gutierrez\}, \{Diana M.\} and Shen Zhang and Aston, \{John E.\} and Thompson, \{David N.\} and Ladisch, \{Michael R.\} and Mosier, \{Nathan S.\}",
note = "Funding Information: Financial support from the United States Department of Energy Bioenergy Technologies Office (DOE-BETO) under contracts DE-EE0008256 and DE-EE0008910 is acknowledged. Work performed at INL was supported by the U.S. Department, Bioenergy Technologies Office, under U.S. Department of Energy Idaho Operations Office Contract No. DE-AC07-05ID14517. This work was also supported by USDA Hatch project IN1016152 “Mitigating Lignin and Other Recalcitrance for Biofuels and Bioproducts from Agriculture”. The authors would like to thank Dr. Eduardo Ximenes of Indiana University-Bloomington at the School of Public Health for helpful discussions and suggestions. Additionally, the authors thank Lindsey R. Crawley and Jennifer A. Rackliffe from LORRE for assistance with HPLC analysis and experimental TKN analysis, respectively, Pankaj Sharma for guiding operations of this multisite research, and Carla Carie for assistance in preparing the manuscript. The views expressed in this paper do not necessarily represent the views of the DOE or the U.S. Government. The publisher, by accepting the article for publication, acknowledges that the U.S. Government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this work, or allow others to do so, for U.S. Government purposes. Publisher Copyright: {\textcopyright} 2023 American Chemical Society.",
year = "2023",
month = dec,
day = "18",
doi = "10.1021/acssuschemeng.3c04222",
language = "English",
volume = "11",
pages = "17616--17624",
journal = "ACS Sustainable Chemistry and Engineering",
issn = "2168-0485",
publisher = "American Chemical Society",
number = "50",
}