Removal of enzymatic and fermentation inhibitory compounds from biomass slurries for enhanced biorefinery process efficiencies

Raghu N. Gurram; Saurav Datta; Yupo J. Lin; Seth W. Snyder; Todd J. Menkhaus

doi:10.1016/j.biortech.2011.05.043

Removal of enzymatic and fermentation inhibitory compounds from biomass slurries for enhanced biorefinery process efficiencies

Raghu N. Gurram, Saurav Datta, Yupo J. Lin, Seth W. Snyder, Todd J. Menkhaus

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Within the biorefinery paradigm, many non-monomeric sugar compounds have been shown to be inhibitory to enzymes and microbial organisms that are used for hydrolysis and fermentation. Here, two novel separation technologies, polyelectrolyte polymer adsorption and resin-wafer electrodeionization (RW-EDI), have been evaluated to detoxify a dilute acid pretreated biomass slurry. Results showed that detoxification of a dilute acid pretreated ponderosa pine slurry by sequential polyelectrolyte and RW-EDI treatments was very promising, with significant removal of acetic acid, 5-hydroxymethyl furfural, and furfural (up to 77%, 60%, and 74% removed, respectively) along with >97% removal of sulfuric acid. Removal of these compounds increased the cellulose conversion to 94% and elevated the hydrolysis rate to 0.69g glucose/L/h. When using Saccharomyces cerevisiae D ₅A for fermentation of detoxified slurry, the process achieved 99% of the maximum theoretical ethanol yield and an ethanol production rate nearly five-times faster than untreated slurry.

Original language	English
Pages (from-to)	7850-7859
Number of pages	10
Journal	Bioresource Technology
Volume	102
Issue number	17
DOIs	https://doi.org/10.1016/j.biortech.2011.05.043
State	Published - Sep 2011
Externally published	Yes

Keywords

Biorenewables
Detoxification
Electrodeionization
Lignocelluosic biomass
Polyelectrolytes

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10.1016/j.biortech.2011.05.043

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title = "Removal of enzymatic and fermentation inhibitory compounds from biomass slurries for enhanced biorefinery process efficiencies",

abstract = "Within the biorefinery paradigm, many non-monomeric sugar compounds have been shown to be inhibitory to enzymes and microbial organisms that are used for hydrolysis and fermentation. Here, two novel separation technologies, polyelectrolyte polymer adsorption and resin-wafer electrodeionization (RW-EDI), have been evaluated to detoxify a dilute acid pretreated biomass slurry. Results showed that detoxification of a dilute acid pretreated ponderosa pine slurry by sequential polyelectrolyte and RW-EDI treatments was very promising, with significant removal of acetic acid, 5-hydroxymethyl furfural, and furfural (up to 77%, 60%, and 74% removed, respectively) along with >97% removal of sulfuric acid. Removal of these compounds increased the cellulose conversion to 94% and elevated the hydrolysis rate to 0.69g glucose/L/h. When using Saccharomyces cerevisiae D 5A for fermentation of detoxified slurry, the process achieved 99% of the maximum theoretical ethanol yield and an ethanol production rate nearly five-times faster than untreated slurry.",

keywords = "Biorenewables, Detoxification, Electrodeionization, Lignocelluosic biomass, Polyelectrolytes",

author = "Gurram, {Raghu N.} and Saurav Datta and Lin, {Yupo J.} and Snyder, {Seth W.} and Menkhaus, {Todd J.}",

note = "Funding Information: Financial support for R. Gurram was provided by the USDA NIFA , AFRI Competitive Grant # 2010-65504-20372 , and the South Dakota School of Mines and Technology . In addition, the work was partially supported by funding from the US Department of Energy, Office of the Biomass Program. The submitted manuscript has been created by UChicago Argonne, LLC, Operator of Argonne National Laboratory (“Argonne”). Argonne, a US Department of Energy Office of Science laboratory, is operated under Contract No. DE-AC02-06CH11357. The US Government retains for itself, and others acting on its behalf, a paid-up nonexclusive, irrevocable worldwide license in said article to reproduce, prepare derivative works, distribute copies to the public, and perform publicly and display publicly, by or on behalf of the Government.",

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T1 - Removal of enzymatic and fermentation inhibitory compounds from biomass slurries for enhanced biorefinery process efficiencies

AU - Gurram, Raghu N.

AU - Datta, Saurav

AU - Lin, Yupo J.

AU - Snyder, Seth W.

AU - Menkhaus, Todd J.

N1 - Funding Information: Financial support for R. Gurram was provided by the USDA NIFA , AFRI Competitive Grant # 2010-65504-20372 , and the South Dakota School of Mines and Technology . In addition, the work was partially supported by funding from the US Department of Energy, Office of the Biomass Program. The submitted manuscript has been created by UChicago Argonne, LLC, Operator of Argonne National Laboratory (“Argonne”). Argonne, a US Department of Energy Office of Science laboratory, is operated under Contract No. DE-AC02-06CH11357. The US Government retains for itself, and others acting on its behalf, a paid-up nonexclusive, irrevocable worldwide license in said article to reproduce, prepare derivative works, distribute copies to the public, and perform publicly and display publicly, by or on behalf of the Government.

PY - 2011/9

Y1 - 2011/9

N2 - Within the biorefinery paradigm, many non-monomeric sugar compounds have been shown to be inhibitory to enzymes and microbial organisms that are used for hydrolysis and fermentation. Here, two novel separation technologies, polyelectrolyte polymer adsorption and resin-wafer electrodeionization (RW-EDI), have been evaluated to detoxify a dilute acid pretreated biomass slurry. Results showed that detoxification of a dilute acid pretreated ponderosa pine slurry by sequential polyelectrolyte and RW-EDI treatments was very promising, with significant removal of acetic acid, 5-hydroxymethyl furfural, and furfural (up to 77%, 60%, and 74% removed, respectively) along with >97% removal of sulfuric acid. Removal of these compounds increased the cellulose conversion to 94% and elevated the hydrolysis rate to 0.69g glucose/L/h. When using Saccharomyces cerevisiae D 5A for fermentation of detoxified slurry, the process achieved 99% of the maximum theoretical ethanol yield and an ethanol production rate nearly five-times faster than untreated slurry.

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KW - Electrodeionization

KW - Lignocelluosic biomass

KW - Polyelectrolytes

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Removal of enzymatic and fermentation inhibitory compounds from biomass slurries for enhanced biorefinery process efficiencies

Abstract

Keywords

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