@article{3eb37ed55d534a9f8b8bb5a9f6799599,
title = "Preservation of yeast cell morphology for scanning electron microscopy using 3.28-μm IR laser irradiation",
abstract = "As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-μm IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.",
keywords = "Dehydration, Electron microscopy, Fixation, IR, Imaging, Infrared laser, Morphology, SEM, Yeast cells",
author = "Jan Sunner and Recep Avci and Lee Richards and Gary Groenewold and Jani Ingram and Megan Arthun",
note = "Funding Information: We wish to thank Jon Eckberg for help in setting up the laser system. Cell cultures were supplied by Nurdan Yurt and Mark Pasmore. The help of ICAL personnel with SEM operations are greatly appreciated. This work was funded by DOE-INRA Contract no. MSU001.",
year = "2003",
month = aug,
day = "1",
doi = "10.1016/S0167-7012(03)00041-1",
language = "English",
volume = "54",
pages = "285--287",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier B.V.",
number = "2",
}