TY - JOUR
T1 - Dissimilatory reduction of Cr(VI), Fe(III), and U(VI) by Cellulomonas isolates
AU - Sani, R.
AU - Peyton, B.
AU - Smith, W.
AU - Apel, W.
AU - Petersen, J.
N1 - Funding Information:
Acknowledgements This research work was supported by the U.S. Department of Energy, Office of Science, Natural and Accelerated Bioremediation Research (NABIR) program by grants DE-FG03-98ER62693, DE-FG03-98ER62630/A001, and DE-FG03-01ER63270 to Washington State University and under DOE Idaho Operations Office Contract DE-AC07-99ID13727. We would like to acknowledge the work of collaborator James E. Amonette at the Pacific Northwest National Laboratory for the MINTEQA2 modeling of U(VI)-carbonate complexes. The support of the WSU/NSF Center for Multiphase Environmental Research and the Department of Chemical Engineering also contributed significantly to this research.
PY - 2002/10
Y1 - 2002/10
N2 - The reduction of Cr(VI), Fe(III), and U(VI) was studied using three recently isolated environmental Cellulomonas sp. (WS01, WS18, and ES5) and a known Cellulomonas strain (Cellulomonas flavigena ATCC 482) under anaerobic, non-growth conditions. In all cases, these cultures were observed to reduce Cr(VI), Fe(III), and U(VI). In 100 h, with lactate as electron donor, the Cellulomonas isolates (500 mg/l total cell protein) reduced nitrilotriacetic acid chelated Fe(III) [Fe(III)-NTA] from 5 mM to less than 2.2 mM, Cr(VI) from 0.2 mM to less than 0.001 mM, and U(VI) from 0.2 mM to less than 0.12 mM. All Cellulomonas isolates also reduced Cr(VI), Fe(III), and U(VI) in the absence of lactate, while no metal reduction was observed in either the cell-free or heat-killed cell controls. This is the first report of Cellulomonas sp. reducing Fe(III) and U(VI). Further, this is the first report of Cellulomonas spp. coupling the oxidation of lactate, or other unknown electron donors in the absence of lactate, to the reduction of Cr(VI), Fe(III), and U(VI).
AB - The reduction of Cr(VI), Fe(III), and U(VI) was studied using three recently isolated environmental Cellulomonas sp. (WS01, WS18, and ES5) and a known Cellulomonas strain (Cellulomonas flavigena ATCC 482) under anaerobic, non-growth conditions. In all cases, these cultures were observed to reduce Cr(VI), Fe(III), and U(VI). In 100 h, with lactate as electron donor, the Cellulomonas isolates (500 mg/l total cell protein) reduced nitrilotriacetic acid chelated Fe(III) [Fe(III)-NTA] from 5 mM to less than 2.2 mM, Cr(VI) from 0.2 mM to less than 0.001 mM, and U(VI) from 0.2 mM to less than 0.12 mM. All Cellulomonas isolates also reduced Cr(VI), Fe(III), and U(VI) in the absence of lactate, while no metal reduction was observed in either the cell-free or heat-killed cell controls. This is the first report of Cellulomonas sp. reducing Fe(III) and U(VI). Further, this is the first report of Cellulomonas spp. coupling the oxidation of lactate, or other unknown electron donors in the absence of lactate, to the reduction of Cr(VI), Fe(III), and U(VI).
UR - https://www.scopus.com/pages/publications/0037209667
U2 - 10.1007/s00253-002-1069-6
DO - 10.1007/s00253-002-1069-6
M3 - Article
C2 - 12382063
AN - SCOPUS:0037209667
SN - 0175-7598
VL - 60
SP - 192
EP - 199
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 1-2
ER -